Journal: Frontiers in Pharmacology

Article Title: Protective effect of Astragaloside IV on chronic intermittent hypoxia-induced vascular endothelial dysfunction through the calpain-1/SIRT1/AMPK signaling pathway

doi: 10.3389/fphar.2022.920977

Figure Lengend Snippet: Effects of As-IV and Capn1 knockout on the SIRT1/AMPK/eNOS signaling pathway in CIH-induced mice. (A–F) Western blot analysis of calpain-1, SIRT1, Thr 172 AMPK phosphorylation, t-AMPK, Ser 1177 eNOS phosphorylation and eNOS protein expression in all groups with quantification ( n = 3). (G–J) Representative fluorescence images and fluorescence intensity of calpain-1 and SIRT1 in all groups of aortic sections (×400 magnification; n = 3). Calpain-1 (green), SIRT1 (red), Hoechst 33342 (blue). Data are presented as means ± SD. #P < 0.05, vs. Con group, ** p < 0.01, *P < 0.05, vs. CIH group.

Article Snippet: Ser 1177 eNOS phosphorylation, total endothelial nitric oxide (eNOS), Thr 172 AMPK phosphorylation, total 5’ AMP activated protein kinase (t-AMPK), vascular cell adhesion molecule 1 (VCAM-1) and intracellular adhesion molecule 1 (ICAM-1) were obtained from ABclonal (Wuhan, China).

Techniques: Knock-Out, Western Blot, Expressing, Fluorescence

Journal: Frontiers in Pharmacology

Article Title: Protective effect of Astragaloside IV on chronic intermittent hypoxia-induced vascular endothelial dysfunction through the calpain-1/SIRT1/AMPK signaling pathway

doi: 10.3389/fphar.2022.920977

Figure Lengend Snippet: Effects of As-IV, MDL-28170 and SRT1720 administration on calpain-1, SIRT1, AMPK, and eNOS protein expression in HCAECs. (A–F) The protein expression levels of calpain-1, SIRT1, Thr 172 AMPK phosphorylation, t-AMPK, Ser 1177 eNOS phosphorylation and eNOS were analyzed using Western blots ( n = 3). Data are presented as means ± SD. ** p < 0.01, *P < 0.05, vs. CIH group.

Article Snippet: Ser 1177 eNOS phosphorylation, total endothelial nitric oxide (eNOS), Thr 172 AMPK phosphorylation, total 5’ AMP activated protein kinase (t-AMPK), vascular cell adhesion molecule 1 (VCAM-1) and intracellular adhesion molecule 1 (ICAM-1) were obtained from ABclonal (Wuhan, China).

Techniques: Expressing, Western Blot

Journal: Nucleus

Article Title: Nucleolar aggresomes mediate release of pericentric heterochromatin and nuclear destruction of genotoxically treated cancer cells

doi: 10.1080/19491034.2017.1279775

Figure Lengend Snippet: Primary antibodies and their source.

Article Snippet: Catalog Nr., Manufacturer Bromo-deoxyuridine Mouse monoclonal Detects BrdU incorporated into DNA as well as bromouridine incorporated into RNA 1:200 {"type":"entrez-nucleotide","attrs":{"text":"A21300","term_id":"514164","term_text":"A21300"}} A21300 , Invitrogen Centromere Protein Human Derived from human CREST patient serum 1:50 15–234, Antibodies Inc. CHK2 (phospho T68) Rabbit polyclonal Epitope around the phosphorylation site of Threonine 68 (VSTpQE) of human Chk2 1:100 ab38461, Abcam Fibrillarin Mouse monoclonal Corresponding to Saccharomyces cerevisiae Fibrillarin 1:50 ab4566, Abcam Histone H3K4me3 Mouse monoclonal Synthetic peptide corresponding to Human Histone H3 aa 1–100 (tri methyl K4) conjugated to KLH 1:50 ab1012 Histone H3K9me3 Rabbit polyclonal Synthetic peptide within Human Histone H3 aa 1–100 (N-terminal) (tri methyl K9) conjugated to KLH 1:400 ab8898 LAMIN B1 Rabbit polyclonal Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of mouse Lamin B1 1:200 ab16048, Abcam LAMP2 Mouse monoclonal The details of the immunogen for this antibody are not available 1:500 555803, BD PharMingen™ LC3B Rabbit polyclonal Peptide derived from within residues 1 - 100 of Human LC3B 1:100 ab63817, Abcam 5′methyl-cytosine Mouse monoclonal Recognizes 5-methylcytosine in methylated DNA 1:100 NA81, Calbiochem p16INK4a Rabbit polyclonal Corresponding to C-terminus of human p16 1:100 ab7962, Abcam p-AMPKα1/2 (Thr183/172) Rabbit polyclonal Epitope corresponding to phosphorylated Thr172 of AMPKα1 of human origin 1:50 sc-101630, Santa Cruz p53(SP5) Rabbit monoclonal Recombinant human full-length wild type p53 protein 1:50 MA5–14516, Pierce RPA194 Mouse monoclonal Raised against amino acids 1–300 of RPA194 of human origin 1:50 sc-48385, Santa Cruz SQSTM1 (p62) Rabbit polyclonal Epitope corresponding to amino acids 151–440 of SQSTM1 of human origin.

Techniques: Derivative Assay, Methylation, Recombinant